{"title":"Z-MAB® Next Generation Control Antibody","description":"\u003cp\u003e\u003cimg alt=\"Z-MAB\"\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 99.941%; height: 392px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"25%\" style=\"width: 16.7721%; height: 19.6px;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"25%\" style=\"width: 32.7217%; height: 19.6px;\"\u003e\u003cstrong\u003eAntibody Isotype   \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"25%\" style=\"width: 24.4789%; height: 19.6px;\"\u003e\u003cstrong\u003eFc Domain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"25%\" style=\"width: 25.0149%; height: 19.6px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003eCatalog Code\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eHuman\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-human-igg1\"\u003ePZHU005\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eHuman\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-human-igg1-with-aglyco-fc\"\u003ePZHU006\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eHuman\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAfucosylated\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003ePZHU005-AF\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eHuman\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG4\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-human-igg4\"\u003ePZHU003\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eHuman\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG4\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-human-igg4-with-aglyco-fc\"\u003ePZHU004\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-mouse-igg1\"\u003ePZMU003\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-mouse-igg1-with-aglyco-fc\"\u003ePZMU004\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAfucosylated\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003ePZMU003-AF\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2a\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-mouse-igg2a\"\u003ePZMU001\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2a\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-mouse-igg2a-with-aglyco-fc\"\u003ePZMU002\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2a\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAfucosylated\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003ePZMU001-AF\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2b\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-mouse-igg2b\"\u003ePZMU005\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eMouse\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2b\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\n\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-mouse-igg2b-with-aglyco-fc\"\u003ePZMU006\u003c\/a\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eRat\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-rat-igg1\"\u003e\u003cspan class=\"variant-sku\"\u003ePZRA001\u003c\/span\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eRat\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-rat-igg1-with-aglyco-fc\"\u003e\u003cspan class=\"variant-sku\"\u003ePZRA002\u003c\/span\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eRat\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2a\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-rat-igg2a\"\u003ePZRA003\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eRat\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2a\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\n\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-rat-igg2a-with-aglyco-fc\"\u003ePZRA004\u003c\/a\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eRat\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2b\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eWildtype\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\u003ca href=\"https:\/\/ab-biosciences.myshopify.com\/products\/z-mab-rat-igg2b\"\u003ePZRA005\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 16.7721%; height: 19.6px;\"\u003eRat\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.7217%; height: 19.6px;\"\u003eIgG2b\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.4789%; height: 19.6px;\"\u003eAglycosylated\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25.0149%; height: 19.6px;\"\u003e\n\u003ca href=\"https:\/\/abbiosciences.com\/products\/z-mab-rat-igg2b-with-aglyco-fc\"\u003ePZRA006\u003c\/a\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eZ-MAB\u003csup\u003e®\u003c\/sup\u003e– A next generation control antibody reagent, Z-MAB\u003csup\u003e®\u003c\/sup\u003e stands for \u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003eZ\u003c\/span\u003eero-binding \u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003em\u003c\/span\u003eonoclonal \u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003eA\u003c\/span\u003enti\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003eb\u003c\/span\u003eody.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eWhat is a Z-MAB\u003csup\u003e®\u003c\/sup\u003e?\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003eZ-MAB\u003csup\u003e®\u003c\/sup\u003e is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB\u003csup\u003e®\u003c\/sup\u003e is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eHow are Z-MAB\u003csup\u003e®\u003c\/sup\u003es created?\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003eStarting with an antibody from a well established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB\u003csup\u003e®\u003c\/sup\u003es exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eWhat unmet needs are Z-MAB\u003csup\u003e®\u003c\/sup\u003es meant to serve?\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB\u003csup\u003e®\u003c\/sup\u003e has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eHow can Z-MAB\u003csup\u003e®\u003c\/sup\u003es help antibody-based research and drug development?\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003eOur Z-MAB\u003csup\u003e®\u003c\/sup\u003e product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB\u003csup\u003e®\u003c\/sup\u003es, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB\u003csup\u003e®\u003c\/sup\u003e was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method.  Human and rat Z-MAB\u003csup\u003e®\u003c\/sup\u003es were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB\u003csup\u003e®\u003c\/sup\u003es of the same species share the same CDR-silenced variable regions. With the Z-MAB\u003csup\u003e®\u003c\/sup\u003es control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB\u003csup\u003e®\u003c\/sup\u003es are particularly useful in animal disease models for antibody drug development where the use of\u003cspan\u003e \u003c\/span\u003eunqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eWhat Z-MAB\u003csup\u003e®\u003c\/sup\u003es are available?\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003eCurretnly, we offer a total of 16 Z-MAB\u003csup\u003e®\u003c\/sup\u003es of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB\u003csup\u003e®\u003c\/sup\u003es, Z-MAB\u003csup\u003e®\u003c\/sup\u003es carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","products":[{"product_id":"z-mab-human-igg1","title":"Human Control Antibody IgG1 Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced human IgG1 antibody with a wildtype Fc domain, PZHU005 binds to human Fc receptors in the following orders (high to low affinity): FcγRI \u0026gt; FcRn \u0026gt; FcγRIIA [H133] ~ FcγRIIA [R133] \u0026gt; FcγRIIIA [V158] ~ FcγRIIIA [F158] \u0026gt; FcγRIIIB ~ FcγRIIB ~ FcγRIIC.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37723819770033,"sku":"PZHU005","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37723819802801,"sku":"PZHU005","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":37723819835569,"sku":"PZHU005","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":46065793630385,"sku":null,"price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":46065793859761,"sku":null,"price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZHU005_Product_Image.png?v=1755475596"},{"product_id":"z-mab-human-igg1-with-aglyco-fc","title":"Human Control Antibody IgG1 Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced human IgG1 antibody with an aglycosylated Fc domain (N297A), PZHU006 exhibits attenuated bindings to human Fcγ receptors, but its binding to human FcRn is not affected\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740405424305,"sku":"PZHU006","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":42877278224561,"sku":"PZHU006","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065799659697,"sku":"PZHU006","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740405457073,"sku":"PZHU006","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":37740405489841,"sku":"PZHU006","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZHU006_Product_Image.png?v=1755475749"},{"product_id":"z-mab-human-igg4","title":"Human Control Antibody IgG4 Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced human IgG4 antibody with a wildtype Fc domain, PZHU003 binds to human FcγRI with high affinity and to FcγRIIA [H131], FcγRIIA [R131], FcγRIIB, FcγRIIC, FcγRIIIA [V158], and FcγRIIIA [F158] with similarly low affinities.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740429476017,"sku":"PZHU003","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740429508785,"sku":"PZHU003","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065801724081,"sku":"PZHU003","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740429541553,"sku":"PZHU003","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877305749681,"sku":"PZHU003","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZHU003_Product_Image.png?v=1755475781"},{"product_id":"z-mab-human-igg4-with-aglyco-fc","title":"Human Control Antibody IgG4 Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced human IgG4 antibody with an aglycosylated Fc domain (N297A), PZHU004 exhibits attenuated bindings to human Fcγ receptors, but its binding to human FcRn is not affected.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740437930161,"sku":"PZHU004","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740437962929,"sku":"PZHU004","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065805623473,"sku":"PZHU004","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740437995697,"sku":"PZHU004","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877318824113,"sku":"PZHU004","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZHU004_Product_Image.png?v=1755475817"},{"product_id":"z-mab-mouse-igg1","title":"Mouse Control Antibody IgG1 Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced mouse IgG1 antibody with a wildtype Fc domain, PZMU003, a mouse IgG1 antibody, can be recycled through binding to FcRn.PZMU003 binds to mouse FcγRIIb and FcγRIII with lower affinity, it does not bind to mouse FcγRI and FcγRIV.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740469878961,"sku":"PZMU003","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740469944497,"sku":"PZMU003","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065806180529,"sku":"PZMU003","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740469977265,"sku":"PZMU003","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877319708849,"sku":"PZMU003","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZMU003_Product_Image.png?v=1755475958"},{"product_id":"z-mab-mouse-igg1-with-aglyco-fc","title":"Mouse Control Antibody IgG1 Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced mouse IgG1 antibody with an aglycosylated Fc domain (N297A), PZMU004 binds FcRn, but not to Fcγ receptors.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740510707889,"sku":"PZMU004","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740510740657,"sku":"PZMU004","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065806475441,"sku":"PZMU004","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740510773425,"sku":"PZMU004","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877319970993,"sku":"PZMU004","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZMU004_Product_Image.png?v=1755476015"},{"product_id":"z-mab-mouse-igg2a","title":"Mouse Control Antibody IgG2a Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced mouse IgG2a antibody with a wildtype Fc domain, PZMU001 binds to mouse FcγRI and FcγRIV with high affinity, to FcγRIIb and FcγRIII with lower affinity, and can be recycled through binding to FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s): \u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e \u003cbr\u003eRelated products: \u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg \u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740518113457,"sku":"PZMU001","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740518146225,"sku":"PZMU001","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065806672049,"sku":"PZMU001","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740518178993,"sku":"PZMU001","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877320528049,"sku":"PZMU001","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZMU001_Product_Image.png?v=1755476105"},{"product_id":"z-mab-mouse-igg2a-with-aglyco-fc","title":"Mouse Control Antibody IgG2a Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced mouse IgG2a antibody with an aglycosylated Fc domain (N297A), PZMU002 exhibits attenuated bindings to mouse Fcγ receptors, but its binding to FcRn is not affected.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740523520177,"sku":"PZMU002","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740523552945,"sku":"PZMU002","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065806868657,"sku":"PZMU002","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740523585713,"sku":"PZMU002","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877323870385,"sku":"PZMU002","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZMU002_Product_Image.png?v=1755476195"},{"product_id":"z-mab-mouse-igg2b","title":"Mouse Control Antibody IgG2b Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced mouse IgG2b antibody with a wildtype Fc domain, PZMU005 binds to mouse FcγRI and FcγRIV with high affinity, to FcγRIIb and FcγRIII with lower affinity, and can be recycled through binding to FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740534399153,"sku":"PZMU005","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740534431921,"sku":"PZMU005","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065806934193,"sku":"PZMU005","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740534464689,"sku":"PZMU005","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877325770929,"sku":"PZMU005","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZMU005_Product_Image.png?v=1755476519"},{"product_id":"z-mab-mouse-igg2b-with-aglyco-fc","title":"Mouse Control Antibody IgG2b Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced mouse IgG2b antibody with an aglycosylated Fc domain (N297A), PZMU006 exhibits attenuated bindings to mouse Fcγ receptors, but its binding to FcRn is not affected.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740549505201,"sku":"PZMU006","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740549570737,"sku":"PZMU006","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065807884465,"sku":"PZMU006","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740549636273,"sku":"PZMU006","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877327573169,"sku":"PZMU006","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZMU006_Product_Image.png?v=1755476565"},{"product_id":"z-mab-rat-igg1","title":"Rat Control Antibody IgG1 Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced rat IgG1 antibody with a wildtype Fc domain, PZRA001 is a good control antibody for experiments using rat IgG1 antibody in a mouse model. It binds to mouse but not human FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740579094705,"sku":"PZRA001","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740579127473,"sku":"PZRA001","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065807982769,"sku":"PZRA001","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740579160241,"sku":"PZRA001","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877329244337,"sku":"PZRA001","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZRA001_Product_Image.png?v=1755476628"},{"product_id":"z-mab-rat-igg1-with-aglyco-fc","title":"Rat Control Antibody IgG1 Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced rat IgG1 antibody with an aglycosylated Fc domain (N297A), PZRA002 exhibits attenuated binding activities to Fcγ receptors. It binds to mouse but not human FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740592398513,"sku":"PZRA002","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740592464049,"sku":"PZRA002","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065808015537,"sku":"PZRA002","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740592496817,"sku":"PZRA002","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877329342641,"sku":"PZRA002","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZRA002_Product_Image.png?v=1755476789"},{"product_id":"z-mab-rat-igg2a","title":"Rat Control Antibody IgG2a Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced rat IgG2a antibody with a wildtype Fc domain, PZRA003 is a good control antibody for experiments using rat IgG2a antibody in a mouse model. It binds to mouse but not human FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740605014193,"sku":"PZRA003","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740605046961,"sku":"PZRA003","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065809621169,"sku":"PZRA003","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740605079729,"sku":"PZRA003","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877332553905,"sku":"PZRA003","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZRA003_Product_Image.png?v=1755476850"},{"product_id":"z-mab-rat-igg2b","title":"Rat Control Antibody IgG2b Wildtype Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced rat IgG2b antibody with a wildtype Fc domain, PZRA005 is a good control antibody for experiments using rat IgG2b antibody in a mouse model. It binds to mouse but not human FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740626477233,"sku":"PZRA005","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":37740626510001,"sku":"PZRA005","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065810800817,"sku":"PZRA005","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":37740626542769,"sku":"PZRA005","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877341860017,"sku":"PZRA005","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZRA005_Product_Image.png?v=1755477793"},{"product_id":"z-mab-rat-igg2b-with-aglyco-fc","title":"Rat Control Antibody IgG2b Aglycosylated Fc","description":"\u003cp class=\"firstcopy\"\u003eA CDR-silenced rat IgG2b antibody with an aglycosylated Fc domain (N297A), PZRA006 exhibits attenuated binding activities to Fcγ receptors. It binds to mouse but not human FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":37740636897457,"sku":"PZRA006","price":250.0,"currency_code":"USD","in_stock":false},{"title":"250 µg","offer_id":37740636930225,"sku":"PZRA006","price":750.0,"currency_code":"USD","in_stock":false},{"title":"1 mg","offer_id":46065810899121,"sku":"PZRA006","price":1500.0,"currency_code":"USD","in_stock":false},{"title":"2 mg","offer_id":37740636962993,"sku":"PZRA006","price":2500.0,"currency_code":"USD","in_stock":false},{"title":"10 mg","offer_id":42877342154929,"sku":"PZRA006","price":5000.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZRA006_Product_Image.png?v=1755477844"},{"product_id":"z-mab-rat-igg2a-with-aglyco-fc","title":"Rat Control Antibody IgG2a Aglycosylated Fc","description":"\u003cp style=\"text-align: left;\"\u003eA CDR-silenced rat IgG2a antibody with an aglycosylated Fc domain (N297A), PZRA004 exhibits attenuated binding activities to Fcγ receptors. It binds to mouse but not human FcRn.\u003c\/p\u003e\n\u003cp\u003eInteracting protein(s):\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/fc-receptor-antibodies\"\u003eFc Receptors\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003eRelated products:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/abbiosciences.com\/collections\/biotin-z-mab\"\u003eOther Biotin-Z-MAB\u003csup\u003e®\u003c\/sup\u003es\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eFor orders over 10 mg\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:sales@abbiosciences.com\"\u003eplease inquire here\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eAn antibody does not bind to antigen – the best control antibody commercially available.\u003c\/h3\u003e\n\u003cp\u003eFor the control arm of an antibody study, AB Biosciences has engineered a unique antibody that does not bind to an antigen, yet it retains all other structural elements mediating all effector functions. Such a reagent represents a novel approach to unambiguously demonstrate that a specific biological activity is resulting from its binding to the cognate antigen rather than from its interactions with other proteins such as Fc receptors, complement and other Fc interacting molecules. We call this Z-MAB® for zero-binding monoclonal antibody. As a novel next generation control antibody, Z-MAB® is the only control antibody commercially available carried silenced CDR regions, that is, it is unable to bind to the antigen.\u003c\/p\u003e\n\u003ch3\u003eWhat are these control antibodies and for what applications?\u003c\/h3\u003e\n\u003cp\u003eZ-MAB® is a genetically engineered antibody variant whose antigen-binding capacity has been eliminated. Z-MAB® is suitable to be used as an unbiased control agent for testing specific activities of an antibody of interest.\u003c\/p\u003e\n\u003ch3\u003eHow these control antibodies are engineered?\u003c\/h3\u003e\n\u003cp\u003eStarting with an antibody from a well-established antibody drug, we identified the critical amino acid residues in all 6 complementary determining regions (CDRs), engineered selected combinatorial variants and tested for the reduction\/loss of their antigen-binding activities. Final candidate Z-MAB®s exhibit excellent CMC and pharmacokinetic properties.\u003c\/p\u003e\n\u003ch3\u003eWhat unmet needs these control antibodies meant to serve?\u003c\/h3\u003e\u003cp\u003eChoosing a control antibody is probably the most unscientific component for testing the specific activity of an antibody of interest. Control antibodies commercially available thus far include antibodies with no known antigens, e.g. MOPC21; antibodies raised against antigens of evolutionarily distant species, e.g. anti-KLH; antibodies reactive with a known antigen that is distinct from the antigen of interest. If cross-reactivity or high background is observed, most researchers simply move to the next control antibody available until an “ideal” control antibody is found, that is, until the expected result is observed. Z-MAB® has no active antigen-binding activity, and yet retains the overall antibody structure and conformation. It is therefore a bona fide control agent for testing antibodies.\u003c\/p\u003e\n\u003ch3\u003eHow can these control antibodies help antibody-based research and drug development?\u003c\/h3\u003e\n\u003cp\u003eOur Z-MAB® product line includes the mostly commonly used isotypes of mouse, human and rat origins. For mouse Z-MAB®s, the IgG2a isotype (PZMU001) was engineered from the OKT3 (a mouse anti-human CD3 antibody) by CDR-silencing. The IgG1 isotype Z-MAB® was generated by joining the variable regions of PZMU001 to the constant regions of a mouse IgG1 antibody. Additional mouse antibody isotypes were generated using the same recombinant method. Human and rat Z-MAB®s were similarly engineered from Avastin (a humanized anti-VEGF antibody) and 53.6.72 (a rat anti-mouse CD8-alpha antibody), respectively. As such, all isotype Z-MAB®s of the same species share the same CDR-silenced variable regions. With the Z-MAB®s control agent, one no longer has to deal with the complications associated with poorly characterized “control” antibodies. In this regard, Z-MAB®s are particularly useful in animal disease models for antibody drug development where the use of unqualified control antibodies frequently leads to unexpected efficacy and for FACS analysis where the background staining frequently makes proper gating a challenge.\u003c\/p\u003e\n\u003ch3\u003eWhat control antibodies are available?\u003c\/h3\u003e\n\u003cp\u003eWe offer a total of 16 Z-MAB®s of mouse, human and rat origin (please see the listing above). In addition to the isotype matched Z-MAB®s, Z-MAB®s carrying aglycosylated Fc domain are available as excellent control agents for antibodies whose Fc regions are not glycosylated, enzymatically or recombinantly.\u003c\/p\u003e","brand":"AB Biosciences","offers":[{"title":"50 µg","offer_id":41857706426545,"sku":"PZRA004","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250 µg","offer_id":41857712029873,"sku":"PZRA004","price":750.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":46065810636977,"sku":"PZRA004","price":1500.0,"currency_code":"USD","in_stock":true},{"title":"2 mg","offer_id":41857712062641,"sku":"PZRA004","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":42877341302961,"sku":"PZRA004","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/files\/PZRA004_Product_Image.png?v=1755477739"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0612\/1825\/collections\/ZMAB_collection.jpg?v=1454607479","url":"https:\/\/abbiosciences.com\/collections\/z-mab-old.oembed?page=2","provider":"AB Biosciences","version":"1.0","type":"link"}