B7-H2, also called ICOS ligand, is a member of the co-stimulatory ligand family. B7-H2 interacts with its receptor, ICOS, on activated cell increases cytokine secretion and helper function to B cells. B7-H2 extracellular IgV domain interacts directly with ICOS, yet IgC domain is required for maintaining its structural integrity. P7096F contains both IgV and IgC domains of B7-H2 and is fused with a mouse IgG2a Fc region.
Interacting protein(s): human ICOS
Related products: Checkpoint Proteins
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|Synonym:||CD275, ICOSLG, B7H2, B7RP-1, B7RP1, GL50, ICOS-L, ICOSL, LICOS|
|MW (Calculated):||53,602 daltons|
|MW (SDS-PAGE):||55 Kd|
|Abs 0.1% (=1 mg/ml):||1.019|
B7-H2 bind to ICOS (inducible co-stimulator) which is a member of the B7/CD28 co-stimulator family that plays essential role in the control of T cell-mediated immune responses. B7-H2, like the B7 proteins, contains a membrane distal IgV and membrane-proximal IgC domains in its extracellular regions.
Like B7-1 and B7-2, B7-H2 contains cysteine residues within the intracellular domain suggesting the existence of the disulfide-linked dimers. B7-H2 was shown to be constitutively expressed on the antigen presenting cells and some parenchymal cells and can be up-regulated in the inflammatory sites. Interestingly, while interferon-ϒ induces expression of B7-1, B7-2, and B7-H2 on dendritic cells, TNF and LPS effectively down regulate B7-H2, and up-regulate the B7-1 and B7-2. It appears that ICOS-B7-H2 interaction has the co-stimulatory effects on recently activated Th2, but not Th1 cells. Consistent with this notion, administration of ICOS-Fc fusion protein suppressed Th2-mediated airway hypersensitivity without affecting the Th1-medidatd alterations in airway functions.
ICOS deficient mice exhibits profound deficiency in antibody isotype-switching and germinal center formation. Isotype switching can be restored in these mice by stimulation through CD40, demonstrating the critical role of ICOS-BH-H2 interaction in the CD40-CD40L pathway.
Amino Acid Sequence
- Costimulation of T cells by B7-H2, a B7-like molecule that binds ICOS. Wang S., Zhu G., Chapoval A.I., Dong H., Tamada K., Ni J., Chen L. Blood 96:2808-2813 (2000)
- Characterization of a new human B7-related protein: B7RP-1 is the ligand to the co-stimulatory protein ICOS. Yoshinaga S.K., Zhang M., Pistillo J., Horan T., Khare S.D., Miner K., Sonnenberg M., Boone T., Brankow D., Dai T., Delaney J., Han H., Hui A., Kohno T., Manoukian R., Whoriskey J.S., Coccia M.A. Int. Immunol. 12:1439-1447 (2000)
- Characterization of human inducible costimulator ligand expression and function. Aicher A., Hayden-Ledbetter M., Brady W.A., Pezzutto A., Richter G., Magaletti D., Buckwalter S., Ledbetter J.A., Clark E.A. J. Immunol. 164:4689-4696 (2000)
- CD28-independent induction of experimental autoimmune encephalomyelitis. Chitnis T., Najafian N., Abdallah K.A., Dong V., Yagita H., Sayegh M.H., Khoury S.J. J Clin Invest. 107:575-83 (2001)