Our recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG2a isotype and is dimerized through the disulfide linkage in the hinge region. This fragment is similar to the Fc fragment generated from an intact antibody by papain digestion as originally shown by Porter et al.
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Quick Spec
| Species: | Mouse |
| Catalog No.: | P0000F |
| Tag: | mouse IgG2a Fc fragment only |
| GenBank Accession: | V00798 |
| SwissPro Accession: | P01863 |
| Expression Host: | 293T |
| Construction: | Mouse IgG2a Fc (I214-K447) |
| MW (calculated): | 27,179 daltons |
| MW (SDS-PAGE): | 30 Kd |
| Abs 0.1% (= 1 mg/ml): | 1.207 |
| Purity: | 95% |
Description
Our recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG2a isotype and is dimerized through the disulfide linkage in the hinge region. This fragment is similar to the Fc fragment generated from an intact antibody by papain digestion as originally shown by Porter et al. The recombinant IgG2a Fc fragment binds to all four mouse FcγRs, with high affinity binding toward FcγRI and FcγRIV (Bruhns 2012).
In general, mouse FcγRs bind efficiently to human Ig subclasses, whereas human FcgRs do not, or poorly bind to mouse Ig subclasses. Specifically, human FcγRI binds mouse IgG2a and IgG2b, but not IgG1. Human FcγRIIA binds mouse IgG1, IgG2a, and IgG2b, but not IgG3, and human FcγRIIIB does not bind mouse IgG.
Interestingly, human FcRn has no affinity to mouse IgG1, but its low affinity to mouse IgG2a and IgG2b is sufficient to restore a mouse IgG2-dependent autoimmune arthritis in human FcRn transgenic mice that was deficient in mouse FcRn. Recombinant soluble mouse IgG2a Fc therefore is useful in interrogation of selective human and mouse Fc receptor without the complications associated with the Fab fragment.
Amino Acid Sequence
References
1. Basta M, Van Goor F, Luccioli S, Billings EM, Vortmeyer AO, et al. (2003) F(ab)'2-mediated neutralization of C3a and C5a anaphylatoxins: a novel effector function of immunoglobulins. Nat Med. 9(4):431-438.
2. Petkova SB, Akilesh S, Sproule TJ, Christianson GJ, Al Khabbaz H, et al. (2006) Enhanced half-life of genetically engineered human IgG1 antibodies in a humanized FcRn mouse model: potential application in humorally mediated autoimmune disease. Int Immunol. 18:1759-1769.
3. Jönsson F, Mancardi DA, Kita Y, Karasuyama H, Iannascoli B, Van Rooijen N, Shimizu T, Daëron M, Bruhns P. (2011) Mouse and human neutrophils induce anaphylaxis. J Clin Invest. 121:1484-1496.
4. Bruhns P. (2012) Properties of mouse and human IgG receptors and their contribution to disease models. Blood. 119:5640-5649.