Choosing the right control antibody is essential for ensuring the accuracy and specificity of antibody-based experiments. Traditional isotype controls offer basic validation, but they often fall short when it comes to more complex applications like cell-based assays or in vivo studies. That’s where Z-MAB®, the zero-binding monoclonal antibody from AB Biosciences, redefines what a control antibody should be.
Engineered for Specificity – Without Off-Target Binding
Z-MAB® antibodies are designed with recombinantly silenced CDRs, ensuring they do not bind any target antigen, yet fully retain native Fc structure and function. Unlike standard isotype controls that may still bind off-target molecules in cells or tissues, Z-MAB® antibodies minimize background and false signals across assays—from flow cytometry to immunoprecipitation and animal studies.
Species and Isotype Matched
Z-MAB® controls are available in species- and isotype-matched formats, which is critical for Fc receptor compatibility and for accurate interpretation of results using species-specific secondary antibodies. This is especially important in cell signaling, ADCC/CDC assays, and immune profiling in both human and animal models.
Validated for Structural Intactness
Each Z-MAB® antibody is rigorously QC-tested using reducing and non-reducing SDS-PAGE to confirm protein integrity. This ensures reliable performance in Fc receptor binding, complement activation, and accurate epitope recognition.
Glycan-Matched Formats for Functional Accuracy
Fc glycosylation—specifically at Asn297—is crucial for effector functions. Z-MAB® antibodies are offered in glycosylated and aglycosylated forms to match your testing antibody’s effector profile, enabling precise comparisons in functional assays.
Endotoxin Controlled and Aggregation-Free
All Z-MAB® antibodies are purified to low endotoxin levels, reducing inflammation artifacts in cell-based or in vivo models. They are also verified for homogeneity by SEC to eliminate aggregates that could skew receptor interactions.
Consistent Formulation
Formulated in isotonic, neutral pH buffers, Z-MAB® antibodies mirror the formulation of standard testing antibodies, eliminating buffer-related variables in functional studies.
Z-MAB® is more than an isotype control—it's the gold standard for functional antibody validation. Shop Z-MAB® control antibodies