CD40 ligand (CD40L or CD154), a type II membrane glycoprotein and a member of the TNF superfamily, is known for its transient expression on activated CD4+ T lymphocytes which is responsible for the helper T cells’ function on resting B cells in a non-antigen-dependent as well as non-major histocompatibility complex-restricted fashions.
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|Synonym:||CD154, gp39, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP|
|Construction:||His-FLAG-Human CD40L (G116-L261)|
|MW (calculated):||18,282 daltons|
|MW (SDS-PAGE):||21 Kd|
|Abs 0.1% .(= 1 mg/ml):||0.797|
CD40 ligand (CD40L or CD154), a type II membrane glycoprotein and a member of the TNF superfamily, is known for its transient expression on activated CD4+ T lymphocytes which is responsible for the helper T cells’ function on resting B cells in a non-antigen-dependent as well as non-major histocompatibility complex-restricted fashions. Interaction of CD40L with its receptor CD40 induces proliferation of and isotype switching in B lymphocytes.
The CD40L gene is located in the X-chromosome. Its mutations of this gene, though rare, is known to be responsible for the X-linked hyper-IgM syndrome in human. In addition to its expression on activated T cells, CD40 ligand can be efficiently translocated the surface of activated platelets. The co-expression of the CD40L and the FcγRIIA on the surface of the activated platelets has been implicated for the thrombogenic activity of the anti-CD40L antibody in human.
CD40L is produced as homotrimeric membrane anchored protein each of the three membrane proximal stalk regions can be sequentially proteolyzed to generate the soluble trimeric protein which retains full binding activity to its receptor, CD40.
Amino Acid Sequence
1. Lederman S., Yellin M. J., Inghirami G., Lee J. J., Knowles D. M., Chess L. (1992) Molecular interactions mediating T-B lymphocyte collaboration in human lymphoid follicles. Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help. J. Immunol. 149, 3817–3826
2. Hsu YM, Lucci J, Su L, Ehrenfels B, Garber E, Thomas D. (1997) Heteromultimeric complexes of CD40 ligand are present on the cell surface of human T lymphocytes. J Biol Chem. 272(2):911-915.
3. Robles-Carrillo L, Meyer T, Hatfield M, Desai H, Dávila M, Langer F, Amaya M, Garber E, Francis JL, Hsu YM, Amirkhosravi A. (2010) Anti-CD40L immune complexes potently activate platelets in vitro and cause thrombosis in FCGR2A transgenic mice. J Immunol. 185(3):1577-1583