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4-1BBL (ΔG-Fc-Fusion)

4-1BBL (ΔG-Fc-Fusion)

$ 415.00

The 4-1BBL can induce anti-tumor activities by promoting CD8 T cell expansion and protect against autoimmunity and transplantation rejection by depletion NK and B cells. The 4-1BB/4-1BBL pathway is a potential therapeutic target for oncology and autoimmune disorders.

Interacting protein(s): 4-1BB
Related products: Hexa-Ligand

Read all details below including Quick Specs.

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Quick Spec

Species: Human
Catalog No.: P7118G
Synonym: CD137L, TNFSF9
Tag: Aglycosylated mouse IgG2a Fc
GenBank Accession: NM_003811
SwissPro Accession: P41273
Expression Host: 293T
Construction: Aglycosylated mouse IgG2a Fc-GIL-Human 4-1BBL (A50–E254)-AAL
MW (calculated): 48287 daltons
MW (SDS-PAGE): 52 Kd
Abs 0.1% (= 1 mg/ml): 1.147
Purity: 95 %

Description

Murine 4-1BB ligand was initially cloned by expression cloning form a cDNA library of EL4 thymoma cells using 4-1BB-Fc fusion protein. The 4-1BB Ligand is a member of the TNF ligand superfamily (TNFSF). It carboxyl terminal extracellular domain contains three N-glycosylation sites. In addition, its membrane proximal region is rich in threonine, serine and proline residues which is indicative of O-linked glycosylation site.

Interestingly, both human and mouse 4-1BB ligand are among the least homologous member of the TNFSF family. While earlier studies indicated that 4-1BB ligand might be an exception within the TNFSF and exists as dimeric protein. Subsequent studies shows the soluble trimeric ligand interacts with 4-1BB just like other TNFSF/TNFRSF pairs.

The anti-tumor effect of the 4-1BB/4-1BBL axis is based on the observation that 4-1BBL stimulates activated T cells, particularly CD8 T cells and induces secretion of high levels of IFN-g even though the potential adverse effects of inducing autoimmune status has to be considered at all time. Interestingly, in vivo administration of agonistic anti-4-1BB antibody leads to depletion of B, NK, and CD4 T cells. Hence, 4-1BBL can promote CD8 T cell expansion as well as protect against autoimmune and transplantation rejection. The 4-1BB/4-1BBL pathway is an important therapeutic candidate for oncology.

Amino Acid Sequence

References

1. Goodwin RG, Din WS, Davis-Smith T, Anderson DM, Gimpel SD, et al. (1993) Molecular cloning of a ligand for the inducible T cell gene 4-1BB: a member of an emerging family of cytokines with homology to tumor necrosis factor. Eur J Immunol. 23:2631-2641.
2. Alderson MR, Smith CA, Tough TW, Davis-Smith T, Armitage RJ, et al. (1994) Molecular and biological characterization of human 4-1BB and its ligand. Eur J Immunol. 24:2219-2227.
3. Sun Y, Chen HM, Subudhi SK, Chen J, Koka R, Chen L, Fu YX. (2002) Costimulatory molecule-targeted antibody therapy of a spontaneous autoimmune disease. Nat Med. 1405-1413.
4. Agarwal A, Newell KA. (2008) The role of positive costimulatory molecules in transplantation and tolerance. Curr Opin Organ Transplant. 13:366-372


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