CD27 (Fc-Fusion)

CD27 is found on the surface of majority of blood T cells, both on the CD4 and CD8 subsets and is defined by a set of monoclonal antibodies. Antigen-activated T cells up-regulate the CD27 expression and CD27 interaction with its receptor augments Ag-receptor mediated T cell proliferation.

Interacting protein(s): CD27L (P7004F)
Related products: TNF-Receptor Superfamily

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Quick Specs

Description

CD27 is found on the surface of majority of blood T cells, both on the CD4 and CD8 subsets and is defined by a set of monoclonal antibodies. Antigen-activated T cells up-regulate the CD27 expression and CD27 interaction with its receptor augments Ag-receptor mediated T cell proliferation. Cloning of CD27 was accomplished by expression cloning using anti-CD27 mAbs from a human PBL cDNA library.

The CD27 protein extracellular domain contains 2 CRDs that exhibit similar sub-domain motifs to those of the CD40 and TNFR2. Similar to human NFGR, CD27’s membrane proximal region is over-represented by Pro, Ser and Thr amino acid residues which are often the O-linked glycosylation sites. This is confirmed by its slower mobility on SDS-PAGE and a shift of isoelectric point (pI) that would be otherwise predicted. CD27’s intracellular domain is relative short and does not have a death domain (DD). However, it interact with SIVA which does contains a DD. Overexpression of SIV triggers cell death suggesting potential role of SIVA in mediating CD27 transduced apoptotic signal.

Amino Acid Sequence.

References

1. Gravestein LA, Blom B, Nolten LA, de Vries E, van der Horst G, et al. (1993) Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family. Eur J Immunol. 23:943-950.
2. Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, et al. (1997) CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Natl. Acad. Sci. U.S.A. 94:6346–6351.
3. Yamamoto, H, Kishimoto T, Minamoto S (1998) NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27. J. Immunol. 161:4753–4759.